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. 2016 Apr 5;11(4):e0152837. doi: 10.1371/journal.pone.0152837

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© 2016 Li et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Ovarian tissues from patients with PCOS and healthy controls were analyzed for differential protein expression by 2D-DIGE. A total of 10 samples were analyzed in each group. Individual samples were labeled with Cy3 (A) and Cy5 (B). Pooled samples were labeled with Cy-2 as an internal control. The three labeled samples were then mixed and loaded into a 2D-PAGE gel for two dimension separation. The labeled proteins were visualized and scanned for imaging. An overlay of three dye-scanned images was obtained (D). One representative result from 10 individual samples is shown.