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. Author manuscript; available in PMC: 2017 Mar 28.
Published in final edited form as: Cancer Lett. 2016 Jan 12;372(2):192–200. doi: 10.1016/j.canlet.2015.12.011

Table 2.

IHC and IF Antibodies dilutions with experimental conditions

Antibody Host Source Cat# Blocking 1 Ab Dilution 2 Ab Dilution
CD31 Rabbit Abcam ab28364 30min 5% goat serum 1:100 1:500 Anti-Rabbit in goat
KI67 Mouse Origene UM800033 30mins,10% Horse serum 1:200 1:500 anti-mouse in horse
CXCR2 Goat Dr. Strieter's lab NA 30min 10% horse serum 1:1000 1:250 anti-goat Cy3 in donkey
Vimentin Goat Dr. Keith Johnson SC-7557 30min 10% horse serum 1:200 1:250 anti-goat Cy3 in donkey
E-cadherin Mouse Dr. Keith Johnson NA 30min 5% goat serum 1:5 1:400 Cy3 anti-mouse in goat
N-cadherin Mouse Dr. Keith Johnson NA 30min 5% goat serum undiluted 1:400 Cy3 anti-mouse in goat
P-cadherin Mouse Dr. Keith Johnson NA 30min 5% goat serum 1:5 1:400 Cy3 anti-mouse in goat
β-catenin Mouse Dr. Keith Johnson NA 30min 5% goat serum undiluted 1:400 Cy3 anti-mouse in goat