Fig 6. Inflammatory genes significantly increased in the thalamus of scrapie-infected mice.
The changes in transcription for 86 inflammatory cytokines and their receptors was analyzed by qRT-PCR and categorized by time of increase (S1 Table for complete list of genes tested). The 11 genes shown here demonstrated a significant increase in expression in either 22L-infected or RML-infected thalamus of mice at the earliest time assessed (40 dpi and 60 dpi, respectively). The solid horizontal line on each graph indicates the mean ΔCT in mock infected mice as a reference. The bars for all samples indicate the mean and 1 standard deviation. The ΔCT for each mouse RNA sample was calculated by subtracting the geometric mean CT of three housekeeping genes (Actin, Gapdh, and Hsp90ab1) from the CT of the gene of interest. Each dot represents an individual mouse.