Table 1. Quantitative analysis by qRT-PCR of fold change of mRNA for specific genes in thalamus of mice microinjected with scrapie strain 22L at 40, 60 and 80 days post-injection.
| 22L 40dpi (n = 8) a | 22L 60dpi (n = 4) | 22L 80dpi (n = 4) | ||
|---|---|---|---|---|
| Gene b | FC c | FC | FC | Description |
| Cxcl10 | 12.9 ** | 35.0 *** | 78.9 *** | Chemokine (C-X-C motif) ligand 10 |
| Ccl4 | 6.9 ** | 11.7 *** | 31.5 *** | Chemokine (C-C motif) ligand 4 |
| IL12b | 2.2 | 11.6 * | 30.6 *** | Interleukin 12 p40 |
| Cxcl9 | 3.2 | 5.4 * | 26.4 *** | Chemokine (C-X-C motif) ligand 9 |
| Cxcl13 | 1.2 | 5.0 *** | 23.9 *** | Chemokine (C-X-C motif) ligand 13 |
| Ccl5 | 3.5 * | 7.5 *** | 18.7 *** | Chemokine (C-C motif) ligand 5 |
| Il1rn | 4.2 * | 7.1 *** | 17.8 *** | Interleukin 1 receptor antagonist |
| Ccl2 | 4.4 ** | 6.1 *** | 15.3 *** | Chemokine (C-C motif) ligand 2 |
| Tnf | 3.7 * | 7.5 ** | 15.2 *** | Tumor necrosis factor |
| Ccl12 | 3.2 ** | 5.8 *** | 13.4 *** | Chemokine (C-C motif) ligand 12 |
| Gfap | 2.3*** | 3.6 *** | 10.9 *** | Glial fibrillary acidic protein |
| Ccl8 | 2.5*** | 4.1 ** | 9.9 *** | Chemokine (C-C motif) ligand 8 |
| Il1b | 4.7 ** | 5.1 *** | 8.8 *** | Interleukin 1 beta |
| Ccl3 | 2.4* | 3.0 *** | 7.9 *** | Chemokine (C-C motif) ligand 3 |
| Ccl9 | 2.4** | 3.5 *** | 6.5 *** | Chemokine (C-C motif) ligand 9 |
| Ccl6 | 2.6*** | 4.2 *** | 6.3 *** | Chemokine (C-C motif) ligand 6 |
| Ccl7 | 3.0 ** | 4.3 ** | 6.3 *** | Chemokine (C-C motif) ligand 7 |
| Osm | 1.5 | 3.6 * | 6.1 ** | Oncostatin M |
| Il27 | 1.5 | 1.2 | 5.4 * | Interleukin 27 |
| Il1a | 1.8** | 2.5*** | 4.2 *** | Interleukin 1 alpha |
| Il2rg | 1.2 | 1.6** | 3.5 *** | Interleukin 2 receptor, gamma chain |
| Aif1 | 1.4 | 1.5 | 3.4 ** | Allograft Inflammatory Factor 1 |
| Gpr84 | 1.3 | 1.8 | 3.3 *** | G protein coupled receptor 84 |
| Ccr5 | 2.8 | 2.7 | 3.3 | Chemokine (C-C motif) receptor 5 |
| Ccr3 | 1.3 | 2.9*** | 3.0 *** | Chemokine (C-C motif) receptor 3 |
| Il2rb | -2.4 | 1.9 | 3.0 *** | Interleukin 2 receptor, beta chain |
| Cxcr3 | 1.1 | 1.9 | 2.9* | Chemokine (C-X-C motif) receptor 3 |
| Il4 | 1.2 | 1.8 | 2.8 | Interleukin 4 |
| Ccr1 | 1.5 | 1.6* | 2.8*** | Chemokine (C-C motif) receptor 1 |
| Il5 | -1.0 | 2.3* | 2.6* | Interleukin 5 |
| Cxcl5 | 1.0 | 2.6* | 2.4** | Chemokine (C-X-C motif) ligand 5 |
| Ccr6 | -1.1 | 1.9 | 2.3 | Chemokine (C-C motif) receptor 6 |
| Cxcl1 | 2.0 | 3.7 *** | 2.3 | Chemokine (C-X-C motif) ligand 1 |
| Ccl11 | 1.2 | 1.8 | 2.3** | Chemokine (C-C motif) ligand 11 |
| Il10ra | 1.0 | 1.3* | 2.2*** | Interleukin 10 receptor, alpha |
a the number of mice per experimental condition.
b Genes shown were found to show a greater than 2.0 fold change at 80 dpi after 22L infection out of 86 neuroinflammatory genes analyzed (S1 Table). Larger bolded numerals highlight genes having a fold change of 3.0 or greater and a P value of <0.05.
c Fold change (FC) relative to mock controls (n = 6). P values are calculated using the Student’s t-test for comparison of the 2(- Delta Ct) values for each gene in control mice versus infected mice.
* P value ≤ 0.05
** P value ≤ 0.01
*** P value ≤ 0.001