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. 2016 Apr 5;11(4):e0151907. doi: 10.1371/journal.pone.0151907

Fig 3. Dibutyryl cAMP suppressed the TGF-α-induced cell migration of WT-HSP20 overexpressed HuH7 cells.

Fig 3

The empty vector- (A) or the WT-HSP20- (B) transfected HuH7 cells were pretreated with the indicated concentrations of dibutyryl cAMP for 1 h, and then stimulated by 3 ng/ml of TGF-α or vehicle for 24 h. The migrated cells were fixed with paraformaldehyde and stained with DAPI for the nucleus (blue signal). The cells were photographed by fluorescent microscopy at a magnification of 20× (upper panel). The migrated cell numbers were counted and plotted as the ratio to the mean of migrated numbers of the cells stimulated by vehicle without dibutyryl cAMP treatment (lower panel). Each value represents the means ± SD (n = 3). *P<0.05, lanes 2, 4 and 6 compared to the values of lanes 1, 3, and 5, respectively. **P<0.05. lanes 4 and 6 compared to the value of lane 2.