Fig 8. The effect of geniposide on Aβ1-42-induced Bax and Bcl-2 proteins abnormally expression level.
Neurons were cultured in the presence of 5 μM oligomeric Aβ1–42 and geniposide (2.5 μM, 5 μM, 10 μM) for 24 h, harvested and the levels of specific proteins were assessed by immunoblot analysis (A). Pretreatment of primary cortical neurons with geniposide (2.5–10 μM) could upregulate Bcl-2 expression (B), downregulate Bax expression (C) and decrease the Bax/Bcl-2 ratio (D) induced by Aβ1–42. NS: non significance. N = 6 per group of cells. Studies were repeated four times and data were expressed as mean ± SEM of percentage of vehicle-treated cells.