Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Apr 4;213(4):555–567. doi: 10.1084/jem.20141388

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Sugawara et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Eosinophils secrete IL-1Ra upon GM-CSF stimulation. (A and B) IL-1Ra in the culture supernatant of small intestinal eosinophils sorted from MyD88-deficient (KO; 10⁵) or littermate control (WT; 10⁵) mice (A) and SPF (5 × 10⁴) or GF (5 × 10⁴) mice (B) after a 24-h culture was measured by ELISA. (C) IL-1Ra in the culture supernatant of bone marrow eosinophils (2 × 10⁴) after a 3 or 5 d culture with acetate (1 mM), butyrate (1 mM), or propionate (1 mM) was measured by ELISA. (D) IL-1Ra in culture supernatant of eosinophils (5 × 10⁴) sorted from small intestine or large intestine was measured by ELISA. (E) IL-1Ra in the culture supernatant of bone marrow eosinophils (2 × 10⁴) after a 3 or 5 d culture with GM-CSF (10 ng/ml), IL-5 (10 ng/ml), IL-33 (10 ng/ml), TGF-β (5 ng/ml), or retinoic acid (RA; 10 mM) was measured by ELISA. (F) IL-1Ra in the culture supernatant of bone marrow eosinophils (1.5 × 10⁴) after a 5 d culture with GM-CSF (10 ng/ml) in the presence of GM-CSF receptor α blocking antibodies (α-GM-CSFRα; 8 µg/ml) or isotype control (Rat IgG2a; 8 µg/ml) was measured by ELISA. (G) Quantitative RT-PCR analysis of GM-CSF in bone marrow, blood, or jejunum (SI). (H) Quantitative RT-PCR analysis of GM-CSF receptor in eosinophils sorted from bone marrow, blood, or small intestine. All data are representative of at least three independent experiments. Bar graphs show the mean ± SEM. *, P < 0.05, ***, P < 0.001; NS, not significant (unpaired Student’s t test).