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. 2016 Apr 4;213(4):621–641. doi: 10.1084/jem.20151182

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© 2016 de Valle et al.

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Figure 6. — Enhanced B cell differentiation is the result of the cell-intrinsic loss of NFκB1. (A–F) mBM chimera mice were generated by mixing ly5.1⁺ WT BM cells with Nfκb1^−/− or WT (both ly5.2⁺) BM cells (1:1 ratio) and transferred into lethally irradiated ly5.1⁺ WT hosts. After 16 wk, spleens were isolated and processed for flow cytometry analysis. Splenocytes were defined on the basis of ly5.2 expression. (A) Ki67 expression in Fo B cells (B220⁺CD21^intIgM^lo); dot plots show GL7 versus Bcl6 staining gated on B220⁺ cells (GCs; GL7⁺Bcl6⁺) and IgG1 staining versus FSC gated on Fo B cells (IgG1⁺ B cells). The numbers represent percentages. (B) Absolute cell numbers of Ki67⁺ Fo B cells, GC B cells, and IgG1⁺ B cells. (C) Expression of Bcl-6 in B cell subsets derived from WT or Nfκb1^−/− BM cells in mBM chimeric mice. (D) Bcl-6 expression (mean fluorescence intensity [MFI]) in Fo and GC B cells. (E) Absolute numbers of WT and Nfκb1^−/− T_FH cells (CD4⁺CXCR5⁺PD-1⁺). (F) Intracellular IL-6 expression in Fo B cells from mBM chimera mice. Results were derived from two independent cohorts of reconstituted mice (n = 6/group). Statistical significance was determined using independent or paired samples Student's t tests as appropriate. *, P < 0.05; **, P < 0.001. ns, not significant.