Table 1.
Amino Acids for Denaturation Analysis | Amino Acids for Orientation Analysis | |||
---|---|---|---|---|
| ||||
Buried (m/z) | Surface (m/z) | Bottom* (m/z) | Top* (m/z) | |
| ||||
BSA | Cys (76.0) | Glu (84.0) | Asn, Tyr (70.0, 107) | His (81.0, 82.0) |
Fgn | Val (83.1) | Pro, Arg (70.1) | - | - |
IgG | Cys (76.0) | Arg (100.1) | Asp, Val (72.0) | Ser (60.0) |
Bottom and top refer to the protein with the orientation shown in figure 5.