Serum levels of 2-methoxyestradiol and CYP1A1 expression in the liver. A, Levels of 2-methoxyestradiol were upregulated in the serum from the hens that were fed whole flaxseed supplemented diet, when analyzed using the Cayman Chemicals EIA assay, n=6. Control vs whole flaxseed (p<0.05), whole flaxseed vs flax oil (p<0.05), B, CYP1A1 enzyme expression increased significantly in the hens that were fed whole flaxseed supplemented diet, analyzed by western blotting, n=5. Letters on the error bar shared among groups indicate no significant difference.