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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Feb 27;1861(5):391–401. doi: 10.1016/j.bbalip.2016.02.022

Figure 2. Effect of lipids on Amplex red oxidation by cytoglobin.

Figure 2

The peroxidase activity of Cygb was studied in the presence of free FAs (AA, OA) or liposomes containing neutral (DOPC) or anionic (DOPA, TOCL, PIP2, PIP3) phospholipids. The oxidation of the peroxidation substrate Amplex red was monitored following the fluorescence of its oxidation product resorufin. 0.5 μM Cygb was incubated with lipids in Cygb/lipid ratios from 1:2.5 to 1:50.