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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Feb 27;1861(5):391–401. doi: 10.1016/j.bbalip.2016.02.022

Figure 4. Effect of thiol-modifying agents on Amplex red oxidation by cytoglobin.

Figure 4

The peroxidase activity of Cygb with an intramolecular disulfide bond (WT, native), with reduced thiols (Cygb, 5mM DTT), with NEM-blocked thiols (Cygb, NEM), or a mutant Cygb without cysteine groups (Cygb, C38S/C83S) was studied using Amplex red as substrate. The reaction was monitored in the absence of lipids or in the presence of OA or anionic phospholids (DOPA, TOCL, PIP2, PIP3). 0.5 μM Cygb was incubated with 12.5 μM of each lipid.