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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: J Immunol. 2016 Mar 7;196(8):3479–3493. doi: 10.4049/jimmunol.1501655

FIGURE 4.

FIGURE 4

Rac1 activation dynamics during fMLP response by macrophages. A) Representative ratiometric images (top panel) of FMLPR.2 cells transiently expressing WT Rac1 biosensor stimulated with 100 nM fMLP for indicated times before fixation, stained for F-actin (middle panel) and mVen emission to confirm biosensor expression (bottom panel); scale bar = 10 μm. B) Quantitation of whole cell levels of Rac1 activity; * p < 0.0001 vs 0 s. C) Quantitation of Rac1 activity at the cell edge by radial edge-erosion analysis, where activity was normalized to the lowest value (center region of the cell) within each time point to show changes in relative edge activity. Black * p < 0.0001 for 30 s and 1 min vs 0 s in 0 – 5.85 μm region; blue * p < 0.02 for 5 min vs 0 s in 0 – 3.9 μm. Data are the mean +/− SEM from 3 independent experiments, ≥ 15 cells/time point/experiment.