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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: J Immunol. 2016 Mar 7;196(8):3479–3493. doi: 10.4049/jimmunol.1501655

FIGURE 7.

FIGURE 7

Optimization of biosensor expression in macrophages. Western blot analysis of A) original (non-optimized) or whitlow-modified Rac1 and Rac2 biosensors transiently expressed overnight. B) Tet-OFF inducible expression of original or whitlow-modified Rac1 and C) Rac2 biosensor. D) Tet-OFF induction of fully optimized, FACS-sorted Rac1, E) Rac2 and F) Cdc42 biosensors. BS = biosensor, Dox = doxycycline at 2 μg/mL, endg = endogenous, P = untransduced parental RAW/LR5 lysate, UT = untransfected.