A. TF-dependent TG (a–b) and FG and FL (c–d) at multiple TF concentrations and single tPA concentration. Increase in TF results in higher thrombin peaks, higher TG velocity peaks, and decreased thrombin lag time (TG parameters). Increased TF also results in greater peak clot density and greater peak clotting velocity, and decreased clot time. Curves were generated from experimental data (n = 3) in normal pooled plasma with 0.13 μg/ml tPA and 0 or 0.14–20 pM TF. B. TF-dependent thrombin generation and fibrin generation and lysis at multiple tPA concentrations and single TF concentration. tPA has no effect on thrombin generation (a) but induces faster lysis with increasing concentration (b). Normal pooled plasma activated with 0.71 pM TF and 0 or 0.03–10 μg/ml tPA was used to induce clot lysis. Curves and shaded areas around the curve depict average values and S.D., respectively, for three independent experiments.