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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2016 Mar 9;159:131–141. doi: 10.1016/j.jsbmb.2016.03.014

Table 1.

Mass spectrometry of primary and secondary products from 20(OH)D3 metabolism by CYP3A4. Mass spectra were recorded as described in the Experimental section. Authentic standards of 20,25(OH)2D3, 20,24R(OH)2D3 and 20,24S(OH)2D3 gave the same parent and daughter ions as the corresponding identified CYP3A4 products.

Product [M + Na]+ [M + K]+ [M + H – H2O]+ [M + H – 2H2O]+ Identification
20,25(OH)2D3 439.1 455.1 399.1 381.1 Dihydroxyvitamin D3
20,24R(OH)2D3 439.1 455.1 399.1 381.1 Dihydroxyvitamin D3
20,24S(OH)2D3 439.1 455.0 399.1 381.1 Dihydroxyvitamin D3
1 (from 20,24R(OH)2D3) 455.4 – 415.4 397.4 Trihydroxyvitamin D3
a (from 20,24S(OH)2D3) 455.4 – 415.4 397.4 Trihydroxyvitamin D3