. Author manuscript; available in PMC: 2017 May 1.

Published in final edited form as: J Steroid Biochem Mol Biol. 2016 Mar 9;159:131–141. doi: 10.1016/j.jsbmb.2016.03.014

Table 3.

Kinetic parameters for production of 20(OH)D3 metabolites by human liver microsomes. Data for K_m and V_max are presented as mean ± standard error of the curve fit. Similar data was obtained in two other experiments.

Product	K_m (μM)	V_max (min⁻¹)	K_m/V_max(μM⁻¹min⁻¹)
H1 (20,25(OH)₂D3)	217 ± 88	134.2 ± 36.0	1.6
H2/H3 A (20,24R(OH)₂D3)	138 ± 49	56.1 ± 11.5	2.5
H2/H3 B (20,26(OH)₂D3)	155 ± 42	34.5 ± 5.5	4.5
H2/H3 C	163 ± 51	24.4 ± 4.6	6.7
H4/H5 A	149 ± 32	14.1 ± 1.8	10.6
H4/H5 B (20,24S(OH)₂D3)	114 ± 31	15.2 ± 2.2	7.5
H6	139 ± 74	21.0 ± 6.5	6.6
Total Products	165 ± 56	292.6 ± 60.3	0.6