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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Mol Cell Neurosci. 2016 Jan 21;72:34–45. doi: 10.1016/j.mcn.2016.01.005

Figure 8. MHCI gene expression in individual P14–18 hippocampal neurons.

Figure 8

A. Table summarizing single-cell RT-PCR data for individual neurons from specific regions of mouse hippocampus.

B. Example gel showing multiplex RT-PCR of cDNA extracted from a single CA1 neuron. Summary. mRNA transcripts for eight MHCI genes were observed in individual CA1 neurons (indicated with ●), while four were observed in granule cells of the dentate gyrus and two were observed in neurons of the CA3 region. Number of individual neurons sampled: CA1, n = 6; Dentate gyrus, n = 2; CA3, n = 3. DG, dentate gyrus; ODC, ornithine decarboxylase (positive control); Arf, ADP-ribosylation factor 1 (positive control); SV, splice variant. “?” indicates RT-PCR not performed on a given sample.

SV1 D splice variant: 818 base pairs (bp) predicted product, 548bp observed, no α1.

SV2 T11 splice variant: 415bp predicted product, 659bp observed, retained second intron.

SV3 T24 splice variant: 736bp predicted product, 184 bp observed, no α2 or α3.

SV 4 Q2 splice variant: 785bp predicted product, 509bp observed, no α3.