Figure 6. HSF-1 controls LET-70 expression.

(A) Expression of indicated GFP reporter in surviving linker cells in him-8(e1489) animals of indicated genotype. **p<0.0001, *p<0.005, Fisher’s exact test. Error bars, SEM. (B) let-70p::let-70::gfp expression in head region after heat shock. Error bars, SD. ns, not significant, Student’s t-test. (C) let-70 promoter sequence alignment across indicated nematodes. Red, conserved nucleotides. (D) Same as (C) but for rpn-3. (E) let-70p::GFP and let-70∆HSE::GFP expression. Error bar, SEM. *p<0.0001, Fisher’s exact test. (F) let-70 and btbd-2 interactions with hsf-1. Error bars, SEM. Number within bars, no. of animals scored. Animals contained qIs56 and him-5(e1490). ND= not determined.

DOI: http://dx.doi.org/10.7554/eLife.12821.014

Figure 6—figure supplement 1. ∆HSE reduces let-70 promoter::let-70::GFP expression in the linker cell.

(A) Male containing an integrated wild-type let-70p::let-70::GFP transgene; inset: higher magnification image of linker cell. Scale bar, 10 μm. Arrow, linker cell. (B) Same as (A) except with ∆HSE.

DOI: http://dx.doi.org/10.7554/eLife.12821.015