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. 2015 Oct 5;35(23):2979–2990. doi: 10.1038/onc.2015.364

Figure 5.

Figure 5

Analysis of HuR targets modulated by NS3/4A overexpression. (a) The levels of selected HuR target mRNAs in control and SUDHL6-NS3/4A (mass culture) cells were detected by ribonucleoprotein (RNP)-IP with anti-HuR antibody, followed by RT–qPCR analysis. Data show mean and s.d. from at least three separate experiments. * and ** indicate values significantly greater (*P<0.05, and **P<0.01) in SUDHL6-NS3/4A cells (black column) over control cells (white column). (b) Western blot analysis showing protein expression of selected HuR targets in whole cell lysates of vector control (V) and SUDHL6-NS3/4A (NS3/4A) cells. Actin was used as a loading control. (c) Telaprevir treatment attenuates the upregulation of CD79A and CD79B protein levels by NS3/4A. SUDHL6 vector control and NS3/4A-overexpressing cells were treated with 10 μM Telaprevir and its vehicle control (DMSO) for 24 h. Protein level of CD79A, CD79B and NS3 was examined by western blot analysis.