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. 2016 Apr 6;7:396. doi: 10.3389/fpls.2016.00396

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Copyright © 2016 Saenchai, Bouain, Kisko, Prom-u-thai, Doumas and Rouached.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phosphate, Zinc, and Iron concentrations in wild type (WT) rice and Ospho1;1 mutant lines plant grown under individual and combinatory ions deficiency stresses. (A,C,E) Present Pi, Zn, and Fe concentrations in the shoots, respectively. (B,D,F) Present Pi, Zn, and Fe concentrations in the roots, respectively. After a 10 days of germination in 1/4 full-strength of Yoshida media, plants were grown for 3 weeks on full-strength Yoshida medium with different ionic concentrations: Complete medium (Ct) or lacking either individual element Pi, Zn, or Fe; double elements PiZn, PiFe, ZnFe, or triple elements PiFeZn. Shoots and roots were harvested separately and the Pi and Zn and Fe were extracted and their concentrations were determined as indicated in material and methods. Pi concentrations are calculated per fresh weight. Zn and Fe concentrations are calculated per dry weight. The experiment was repeated three times. The data are given as means ± SE (n = 9). Asterisk indicates statistical significance, P < 0.05. Double asterisk indicates statistical significance, P < 0.01.