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. 2016 Apr 6;7:396. doi: 10.3389/fpls.2016.00396

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Copyright © 2016 Saenchai, Bouain, Kisko, Prom-u-thai, Doumas and Rouached.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcripts abundance of sense and anti-sense of OsPHO1;1 and OsPHO1;2 in OsPHO1;3 as determined by qRT-PCR. OsActin1 was used as an internal reference. qRT quantitative real-time-PCR was performed to analyze the OsPHO1;1 and OsPHO1;2 in OsPHO1;3 mRNA levels in response to under multiple nutritional stresses: complete Yoshida medium (Ct) or lacking to either individual element phosphate (-Pi), zinc (-Zn), or iron (-Fe); double elements -Pi–Zn, -Pi–Fe, -Zn–Fe, or triple elements -Pi-Fe-Zn. All values are relative to wild-type (WT) roots. The experiment was repeated three times. The data are given as means ± SE (n = 9). Asterisk indicates statistical significance, P < 0.05. Double asterisk indicates statistical significance, P < 0.01.