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. 2016 Mar 31;7:11095. doi: 10.1038/ncomms11095

Figure 3. Overexpression of PHT5 leads to Pi overaccumulation and retarded growth.

Figure 3

The Pi level of the shoots and roots of PHT5-overexpressing lines grown under Pi-sufficient conditions. p35S:PHT5;1-GFP in a, p35S:PHT5;2-HA in b and p35S:PHT5;3-GFP in c. The fresh weight of 21-day-old seedlings (d) and the growth phenotypes of 11-day-old seedlings (e) of PHT5;1-GFP-overexpressing lines. Scale bar, 1 cm. Three independent transgenic lines are shown for each construct. Error bar, s.d. (n=3). Asterisks indicate a significant difference compared with WT, *P<0.05 and **P<0.01, Student's t-test. Results were reproducible in at least three independent experiments.