Figure 8.

Four subtypes of GAD⁺ neurons differ in soma profile area. A, Box and whisker plot showing the soma profile area range and median for GAD⁻ neurons (yellow), as well as each GAD subtype. GAD-only neurons (light blue) are significantly smaller than GAD⁺ neurons in the other groups, whereas GAD–PN–VGLUT2 ring neurons (dark green) are significantly larger than GAD⁺ neurons in other groups. GAD⁻ neurons are significantly smaller than all four groups of GAD⁺ neurons. ***p < 0.001. B, Frequency distributions of soma profile area for each of the GAD⁺ subtypes, as well as GAD⁻ neurons. Vertical red lines indicate the soma size classification scheme proposed in this study for IC GAD⁺ neurons (small, <105 μm²; medium, 105–318 μm²; large, >318 μm²).