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. 2016 Apr 6;36(14):4000–4009. doi: 10.1523/JNEUROSCI.3967-15.2016

Figure 1.

Figure 1.

Monosynaptic RV system labels the direct presynaptic inputs to interneurons infected with both AAV and RV. A, Elements of the monosynaptic RV system. Rosa26-LSL-TVA mice were crossed with either PV-Cre, SST-Cre, or VIP-Cre animals to produce mice expressing Cre and TVA (light blue) in a restricted subset of neurons. AAV8-DIO-HB (green), when injected into cortex, expresses nuclear-localized eGFP and RG only in Cre-expressing interneurons. B, EnvA+RVdG-mCh (magenta) is injected in the same location 3–4 weeks later. This virus can only infect cells expressing TVA. C, Only cells that express both TVA and RG permit retrograde spread of RV. Thus, only cells expressing both H2B-eGFP (coexpressed with RG) and mCherry are true starter cells. One week later, monosynaptic inputs to targeted interneurons are brightly labeled with mCherry.