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. 2016 Apr 6;36(14):4000–4009. doi: 10.1523/JNEUROSCI.3967-15.2016

Figure 5.

Figure 5.

Number of input neurons per starter cell varies across cortical interneuron class. A, Plot of number of input neurons per starter cell for each of the brain regions described in Figure 4A. VIP+ interneurons tend to receive greater numbers of inputs per starter cell than other interneuron types; this is quantified in B–D. Thick bars depict mean numbers of inputs per starter cell, and the thin line indicates +1 SEM. B, Anatomical input magnitude distribution for each interneuron class. Input magnitude is defined as the natural log of the number of input neurons per starter cell, with a small modifier for division of small numbers (see main text). Colored boxes indicate the 25th–75th percentiles. The horizontal line contained in each colored box indicates the median input magnitude for that interneuron class. Whiskers indicate the full extent of the data. When summed across all brain areas, SST input is weaker than VIP input (p < 0.01), with PV input magnitude tending to fall between the other two interneuron types. C, Cortical input magnitude distribution is calculated as in B, but only for cortical inputs. Again, cortical input to SST cells is weaker than that to VIP interneurons (p < 0.01). D, Thalamic input magnitude distribution. Although there was a tendency toward a difference between the three groups (p = 0.07 by one-way ANOVA), variability across mice prevented any post hoc comparison. E, Proportion of deep layer (layer 5–6) input compared with total cortical input. VIP interneurons receive a greater proportion of deep-layer input than SST interneurons (p < 0.01). F, Superficial layer (layer 2–3) cortical input magnitude. Even though VIP+ interneurons received the smallest proportion of superficial layer inputs (Fig. 5E), VIP+ interneurons still received higher levels of superficial input per starter cell than SST+ interneurons (p < 0.05). A single outlier (>2.7σ) in the PV interneuron group is plotted as a black cross. G, Deep layer (layer 5–6) cortical input magnitude. As expected from C and E, VIP interneurons have the greatest deep-layer input strength, followed by PV interneurons, then finally the SST cell population (VIP vs PV, p < 0.05; VIP vs SST, p < 0.01; PV vs SST, p < 0.05).