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. 2016 Mar 23;2016:2586706. doi: 10.1155/2016/2586706

Figure 1.

Figure 1

HSP27 alleviates the aging-induced decline of cardiac function. (a) Expression of HSP27 in the hearts of HSP27 Tg mice. The hearts were collected from adult WT and Tg mice. Protein extracts were prepared for immunoblot analysis against HSP27. The blots against GAPDH served as loading controls. Note that the primary antibody against HSP27 had no cross-reaction with murine homologue of HSP27. n = 3 per group. (b) Representative images of pulse doppler of the bicuspid valve echocardiography. n = 6, 9, 3, and 14 in young WT, young Tg, old WT, and old Tg groups, respectively. (c) E/A ratios. ∗ P < 0.05 and ∗∗ P < 0.01. n = 6, 9, 3, and 14 in young WT, young Tg, old WT, and old Tg groups, respectively. (d) Representative images of M-mode tracing of echocardiography. n = 10, 10, 7, and 38 in young WT, young Tg, old WT, and old Tg groups, respectively. (e) EF, FS, LVVd, and LVVs values. ∗∗ P < 0.01. n = 10, 10, 7, and 38 in young WT, young Tg, old WT, and old Tg groups, respectively.