Figure 4. CARD9 is required for the development of the inflammatory environment.

(a–c) Wild type (WT) or Card9^−/− mice were subjected to K/B × N serum-transfer arthritis as described above and the synovial area was flushed on day 4. The cell-free supernatants of the synovial infiltrates were probed using commercial ELISA assays for the indicated chemokines (a), cytokine (b) and the lipid mediator LTB₄ (c). Graphs represent mean and s.e.m. from 4–9 (a), 3–5 (b) or 3–4 (c) mice per group from three independent experiments. *P<0.05; NS, statistically not significant (two-way ANOVA); see the text for actual P values.