Figure 2. Kinetics of conformational change.

Sampling from the MSM captures conformational changes and the timescales at which they occur. (a) In apo-C-CaM, transition to a holo-like state coincides with aromatic stacking and formation of the second, but not the first Ca²⁺-binding site. (b) Holo-C-CaM experiences fluctuations in the inter-EF hand loop region but does not show sustained fluctuations in the aromatic cluster or either Ca²⁺-binding site. All distances are shown in Å.