Table 2. Sequence detail of T-cell clones shared between CNS and peripheral blood.
| Amino acid | TCRBV | TCRBJ | % of peripheral RE samples | % of peripheral control samples |
|---|---|---|---|---|
| RE-specific common clones | ||||
| CASSLGNQPQHF | 07-09 | 01-05 | 52.17 | 0.00 |
| CASSGYEQYF | 02-01 | 02-07 | 39.13 | 0.00 |
| CASSSSYEQYF | 07-02 | 02-07 | 39.13 | 0.00 |
| CSVEGGSSYEQYF | 29-01 | 02-07 | 39.13 | 0.00 |
| CASSDRDTGELFF | 06-04 | 02-02 | 39.13 | 0.00 |
| CASSDSNTGELFF | 06-04 | 02-02 | 34.78 | 0.00 |
| CASSPSYEQYF* | 07-09 | 02-07 | 30.43 | 0.00 |
| CASSLYNEQFF | 27-01 | 02-01 | 30.43 | 0.00 |
| CASSLGYEQYF | 07-09 | 02-07 | 30.43 | 0.00 |
| CSVETDTQYF | 29-01 | 02-03 | 26.09 | 0.00 |
| CASSRYEQYF | 28-01 | 02-07 | 26.09 | 0.00 |
| CSVGQNTEAFF | 29-01 | 01-01 | 26.09 | 0.00 |
| CSVGTGAYEQYF | 29-01 | 02-07 | 26.09 | 0.00 |
| CASSSDSYEQYF | 07-09 | 02-07 | 26.09 | 0.00 |
| CASSVGGTGELFF | 09-01 | 02-02 | 26.09 | 0.00 |
| CASSDSGTDTQYF | 06-04 | 02-03 | 26.09 | 0.00 |
| CSVGTGGTNEKLFF | 29-01 | 01-04 | 26.09 | 0.00 |
| CASSLGQGNTEAFF | 05-01 | 01-01 | 26.09 | 0.00 |
| CASSDSGGSYNEQFF | 06-04 | 02-01 | 26.09 | 0.00 |
| CASSDSSGSTDTQYF | 06-04 | 02-03 | 26.09 | 0.00 |
| CASSDSSGGADTQYF | 06-04 | 02-03 | 26.09 | 0.00 |
| CATSGRKRTGADTEAFF | 15-01 | 01-01 | 26.09 | 0.00 |
| CASSFNEQFF | 28-01 | 02-01 | 21.74 | 0.00 |
| CSVVEETQYF | 29-01 | 02-05 | 21.74 | 0.00 |
| CASSLGSEAFF | 05-01 | 01-01 | 21.74 | 0.00 |
| CASSFTGELFF | 05-06 | 02-02 | 21.74 | 0.00 |
| CASSYGDGYTF | 06-05 | 01-02 | 21.74 | 0.00 |
| CASSLGETQYF | 05-06 | 02-05 | 21.74 | 0.00 |
| CASSFGETQYF | 07-09 | 02-05 | 21.74 | 0.00 |
| CASSLAYEQYF | 07-02 | 02-07 | 21.74 | 0.00 |
| CASSLAYEQYF | 05-01 | 02-07 | 21.74 | 0.00 |
| CASSLLETQYF | 07-09 | 02-05 | 21.74 | 0.00 |
| CASSLGSQPQHF | 07-09 | 01-05 | 21.74 | 0.00 |
| CASSLGTDTQYF | 05-01 | 02-03 | 21.74 | 0.00 |
| CASSPTGSYEQYF | 07-09 | 02-07 | 21.74 | 0.00 |
| CASSIRSSYEQYF | 19-01 | 02-07 | 21.74 | 0.00 |
| CASSLGGNQPQHF | 13-01 | 01-05 | 21.74 | 0.00 |
| CASSLAGGNEQFF | 07-09 | 02-01 | 21.74 | 0.00 |
| CASSVAGGTDTQYF | 09-01 | 02-03 | 21.74 | 0.00 |
| CASSGTSGSTDTQYF | 06-04 | 02-03 | 21.74 | 0.00 |
| CASSDNEQFF | 28-01 | 02-01 | 17.39 | 0.00 |
| CAWSPNTEAFF | 30-01 | 01-01 | 17.39 | 0.00 |
| CASSADYGYTF | 09-01 | 01-02 | 17.39 | 0.00 |
| CAWSPGTEAFF | 30-01 | 01-01 | 17.39 | 0.00 |
| Non-RE-specific common clones | ||||
| CASSLGGTEAFF | 07-09 | 01-01 | 56.52 | 65.22 |
| CASSLGTGELFF | 07-09 | 02-02 | 21.74 | 39.13 |
| CASSFTDTQYF | 07-09 | 02-03 | 26.09 | 30.43 |
| CASSLAGGYEQYF | 05-01 | 02-07 | 17.39 | 30.43 |
| CASSIGGEQFF | 19-01 | 02-01 | 26.09 | 13.04 |
| CASSLLPSYEQYF | 28-01 | 02-07 | 13.04 | 21.74 |
| CASSAGDTQYF | 25-01 | 02-03 | 13.04 | 17.39 |
| CASSMVRGTEAFF | 19-01 | 01-01 | 13.04 | 13.04 |
| CASSLVTSGYNEQFF | 07-09 | 02-01 | 8.70 | 17.39 |
| CNS-resident RE-specific common clones | ||||
| Shared in 3 RE CNS samples | ||||
| CASSADYGYTF | 09-01 | 01-02 | 17.39 | |
| Shared in 2 RE CNS samples | ||||
| CASSGYEQYF | 02-01 | 02-07 | 39.13 | |
| CASSVGSNYEQYF | 09-01 | 02-07 | 17.39 | |
| CASSVVSTGELFF | 09-01 | 02-02 | 17.39 | |
| CASSETTPADTQYF | 06-01 | 02-03 | 13.04 | |
| CASSPYTGGVLDEQFF | 09-01 | 02-01 | 13.04 | |
| CASSGYDYTF | 02-01 | 01-02 | 8.70 | |
| CASSADAHTQYF | 09-01 | 02-03 | 8.70 | |
| CASSLTHPYDYTF | 07-09 | 01-02 | 8.70 | |
| CASRTTGPNDTQYF | 07-09 | 02-03 | 8.70 | |
| CASSESTGLKTQYF | 06-01 | 02-05 | 8.70 | |
| CASSFVPHSTEAFF | 07-03 | 01-01 | 8.70 | |
| CASSGEGASYNEQFF | 07-09 | 02-01 | 8.70 | |
| CSASRDSLENTEAFF | 20-01 | 01-01 | 8.70 | |
| CASSVGGSPTQPQHF | 09-01 | 01-05 | 8.70 | |
| CASSLAGGEYNEQFF | 07-02 | 02-01 | 8.70 | |
| CASSPRVGLTMSGYTF | 09-01 | 01-02 | 8.70 | |
| CAIRGRQGAFGVWTEAFF | 10-03 | 01-01 | 8.70 | |
| Shared in 1 RE CNS sample | ||||
| CSVGTGGTNEKLFF | 29-01 | 01-04 | 26.09 | |
| CASSLGQGNTEAFF | 05-01 | 01-01 | 26.09 | |
| CASSVAGGTDTQYF | 09-01 | 02-03 | 21.74 | |
| CASSLGPYEQYF | 07-02 | 02-07 | 17.39 | |
| CASSIDREETQYF | 19-01 | 02-05 | 17.39 | |
| CSGGGNEQFF | 29-01 | 02-01 | 13.04 | |
| CASGGNEQFF | 02-01 | 02-01 | 13.04 | |
Listed are public clones with their amino-acid sequence, Vβ and Jβ gene, and in how many RE patients or controls this clonotype has been found. This is shown for RE-specific clones (only found in RE patients), non-RE-specific clones (found in both RE patients and controls), as well as RE-specific clones, which were also present in at least one CNS sample. The CDR3 sequence of a clone published previously in paediatric acquired severe aplastic anaemia is marked by an asterisk. The TCRBV entries highlighted in bold correspond to overexpressed genes in RE-specific clones (please see Figure 4 for details).