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. 2016 Apr 4;7:11168. doi: 10.1038/ncomms11168

Figure 7. The U1 70K protein is partially dispensable in mildly affected exons.

Figure 7

(a) Effect of ExSpeU1 variants on mutant minigenes. Splicing patterns were evaluated and quantified as in Fig. 6. Data are expressed as means±s.d. of three independent experiments performed in duplicate. (b) The histogram shows the activity of the ExSpe U1 mutants relative to the native particles and data are calculated as described in Fig. 6. Data represent the mean of the effect in four minigene systems. (Student's t-test **P≤0.01, ***P≤0.001; NS, not significant). (c) Effect of silencing of U1-specific proteins on native ExSpeU1-induced splicing rescue. siRNA treated and untreated HeLa cells were co-transfected with minigenes and corresponding ExSpeU1s. Data are expressed as mean±s.d. of three independent experiments done in duplicate.