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. 2016 Apr 4;7:11169. doi: 10.1038/ncomms11169

Figure 3. MiR-192 mediates its anti-angiogenic effects through regulating EGR1 and HOXB9.

Figure 3

(a) SKOV3ip1 cells stably expressing empty vector (EV), EGR1, HOXB9 or EGR1+HOXB9, were treated with control miRNA or miR-192. Expression levels of pro-angiogenic factors downstream of EGR1 (EFNA1, CXCL1, FGF2) or downstream of both EGR1 and HOXB9 (IL-6, IL-8 and IL-1β) were assessed at 48 h following transfection (n=3, Student's t-test). (b) Tube formation potential of RF-24 cells was assessed following incubation with conditioned media collected from SKOV3-EV, EGR1, HOXB9 and EGR1+HOXB9 cells treated with control miRNA or miR-192 (48 h post transfection, n=5, Student's t-test). Scale bar, 100 μm. High power field, HPF; all the images were taken at × 200 magnification. All the bars and error bars represent mean values and the corresponding s.e.m. (*P<0.05; **P<0.01; and ***P<0.001).