Table 1. Microtubule gliding assay with TC-engineered DmKHC constructs.
| Construct | Loop targeted | Putative function | MT gliding results* | |
|---|---|---|---|---|
| Absence of FlAsH | Presence of FlAsH | |||
| DmKHC_TC(G30) | L1 | ATP binding | 597±12 nm s−1 | No MT binding |
| DmKHC_TC(G50) | L2 | MT interaction | 582±8 nm s−1 | No MT binding |
| DmKHC_TC(P61) | L3 | ATP binding/hydrolysis | MT binding; no gliding | NA |
| DmKHC_TC(G107) | L5 | ATP binding/hydrolysis | MT binding; no gliding | NA |
| DmKHC_TC(G174) | L8 | MT interaction | MT binding; no gliding | NA |
| DmKHC_TC(P312) | L14 | MT interaction | 113±7 nm s−1 | 106±9 nm s−1 |
MT, microtubule; NA, not applicable.
*Data are presented as the mean±s.e. of the mean. n≥30 microtubules for all conditions.