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. 2016 Apr 6;6:23964. doi: 10.1038/srep23964

Figure 5. Autophagy activity is impaired in AD brain.

Figure 5

(A) Protein lysates were extracted from the frontal cortex of human AD brain of various Braak stages (Braak stages III, IV, V, VI) and their age-matched healthy controls (Braak stage I/II). Protein lysates were subjected to WB analysis with anti-LC3 antibody. Actin was used as a loading control. Each line represents individual patient sample. (B) Quantification and statistical analyses of LC3-II expression in AD brain of various Braak stages and age-matched healthy controls. Data are presented as the mean ± SE of the relative protein level (normalized to controls) of LC3-II. n = 3 in each Braak stage, one-way ANOVA, Bonferroni test, *p < 0.05; **p < 0.01. Cropped blots were presented in (A). The gels have been run under the same experimental conditions. Full-length blots were presented in Supplementary Figure 2.