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. 2016 Apr 6;6:24034. doi: 10.1038/srep24034

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(A) The zebrafish embryo phenotype after trim69 knock-down. trim69-CON(a): control group; trim69-MO(b): trim69 knocking down; trim69-MO:trim69-mRNA(c): co-injection with trim69-MO and human trim69 mRNA; black arrow indicates mid-hind brain boundary(MHB); scale bar: 200 μm; (d) Statistical analysis of the zebrafish embryo phenotype. Normal: the phenotype of the zebrafish embryo is normal; deformed: the phenotype of the zebrafish embryo phenotype is abnormal. (B) The examined apoptosis of zebrafish embryos by TUNEL assay. trim69-CON(e): control group; trim69-MO(f): trim69 knocking down; trim69-MO:trim69-mRNA(g): co-injection with trim69-MO and human trim69 mRNA; black arrow indicates apoptotic cells; scale bar: 200 μm.