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. 2016 Apr 6;6:24034. doi: 10.1038/srep24034

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(A,B) HEK-293T cells were transfected with Flag-TRIM69 and HA-c-Jun. After immunoprecipitation with anti-Flag beads, HA-c-Jun was detected by immunoblotting using an anti-HA antibody; after immunoprecipitation with anti-HA beads, Flag-TRIM69 was detected by immunoblotting using an anti-Flag antibody. (C) HeLa cells were co-transfected with Flag-TRIM69 and HA-c-Jun. Subcellular localization of TRIM69 and c-Jun was analyzed by immuno-fluorescence (IF) with anti-Flag and anti-HA antibodies. Nuclei were stained with Hoechst33258.