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. 2016 Apr 6;6:24055. doi: 10.1038/srep24055

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(a) Examples of microcolonies exhibiting heterogeneous Crimson staining. (b) Growth resumption in microcolony after addition of gluconate and GFP inducer (IPTG). (c) Distribution of Crimson expression levels at 0 h timepoint. Black line separates low and high Crimson populations. (d) Growth resumption of cells with low and high Crimson content. Cells were grouped according to their Crimson expression levels at 0 h timepoint. Values are an average from three independent experiments and error bars indicate s.e.m. Time-courses were analysed using linear regression t-test in Graphpad software package and found to be significantly different from each other (p < 0.0001).