Figure 1. The generation of mice homozygous for a disrupted Ndfip2 gene.

(a) The knockout mice were generated by EUCOMM. Location of the inserted trapping cassette within intron 2 of the Ndfip2 gene, resulting in a truncated transcript that encodes exons 1 and 2. The common forward primer (a), wild type reverse primer (b) and knockout reverse primer (c) are indicated. (b) PCR results from one litter produced by two heterozygous parents showing the sizes of the wild type (558 bp) and knockout (329 bp) bands. WT, wild type; M, marker. (c) Immunoblot showing the absence of Ndfip2 protein in Ndfip2^−/− spleen tissue.