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. 2016 Apr 6;6:24045. doi: 10.1038/srep24045

Figure 4. DMT1 expression is increased in the liver of female Ndfip2−/−mice, and there is no compensation for loss of Ndfip2 by Ndfip1 or Nedd4-2.

Figure 4

(a) Western blot showing levels of Ndfip1, Nedd4-2, DMT1 and Ndfip2 in female and (c) male liver protein lysates. There is no change seen in the levels of Ndfip1 or Nedd4-2 in Ndfip2−/− samples, indicating no compensation is occurring. DMT1 levels are increased in Ndfip2−/− female mice, but unchanged in male mice, suggesting that Ndfip2 is important for liver DMT1 regulation in females. β-actin acts as a loading control. (b,d) Quantitation of DMT1 expression relative to β-actin in liver samples from (a,c) showing significant upregualation of DMT1 in female Ndfip2−/− mice. Data expressed as mean ± s.e.m. (n = 3), *p = 0.04. (e,f) DMT1 expression in liver sections from wild type and Ndfip2−/−female mice showing an increase in DMT1-positive puncta in the Ndfip2−/−liver. (g,h) DMT1 expression in liver sections from male mice showing no significant difference between wild type and Ndfip2−/− mice. Representative images of n = 4.