Table 1. Hematological parameters in male and female mice fed a low or normal iron diet.
Male |
Female |
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Low iron diet |
Normal diet |
Low iron diet |
Normal diet |
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Wild type | Ndfip2−/− | Wild type | Ndfip2−/− | Wild type | Ndfip2−/− | Wild type | Ndfip2−/− | |
Hemoglobin (g/L) | 104.4 ± 11.5 | 111.0 ± 10.6 | 122.0 ± 6.7 | 125.4 ± 9.3 | 102.3 ± 1.5 | 113.2 ± 11.8 | 126.0 ± 8.1* | 128.1 ± 5.8* |
RBC count (x1012/L) | 8.7 ± 0.3 | 8.9 ± 0.5 | 7.5 ± 0.3* | 7.7 ± 0.7* | 8.6 ± 0.3 | 8.9 ± 0.3 | 7.9 ± 0.5* | 8.1 ± 0.4* |
Hematocrit (%) | 35.0 ± 3.0 | 37.0 ± 4.0 | 39.0 ± 1.0 | 40.0 ± 4.0 | 34.7 ± 1.2 | 38.0 ± 4.1 | 41.2 ± 1.7* | 41.1 ± 1.5* |
MCV (fl) | 41.2 ± 2.4 | 41.3 ± 2.6 | 52.2 ± 1.0* | 52.2 ± 0.9* | 40.5 ± 2.2 | 42.8 ± 4.2 | 52.3 ± 1.1* | 51.1 ± 0.9* |
MCH (pg) | 12.3 ± 0.7 | 12.4 ± 0.7 | 16.2 ± 0.4* | 16.3 ± 0.9* | 11.9 ± 0.5 | 12.8 ± 1.2 | 16.0 ± 0.1* | 15.9 ± 0.2* |
MCHC (g/L) | 299.4 ± 9.5 | 302.0 ± 6.2 | 310.8 ± 10.9 | 313.0 ± 19.9 | 294.7 ± 3.5 | 298.8 ± 7.1 | 306.8 ± 7.4* | 310.9 ± 5.6 |
RDW (%) | 22.5 ± 2.7 | 21.9 ± 1.6 | 16.4 ± 1.1* | 16.3 ± 1.0* | 22.3 ± 1.0 | 22.1 ± 1.7 | 15.9 ± 0.2* | 15.9 ± 1.2* |
There are no significant differences in any of these parameters between wild type and knockout mice, or between males and females on any diet. *represents significant differences between low iron and normal diets within genders and genotypes. Data expressed as mean ± s.d.; n = 3–8. RBC; red blood cell; MCV, mean corpuscular volume; MCH, mean cell hemoglobin; MCHC, mean cell hemoglobin concentration; RDW, red cell distribution width.