Table 2. Recommended staining protocols for heart issue on glass slides (4 to 40 μm sections).

	4–8 μm Paraffin Sections	40 μm Paraffin Sections
Dewax and rehydration	3 changes of 2 minutes each–Xylene3 changes of 2 minutes each-Ethanol	3 changes of 10 minutes each–Xylene 3 changes of 10 minutes each-Ethanol
Wash	2 minutes in running water*	10 minutes in running water*
Stain	10 μg/mL WGA-AF488 and1 μg/mL DAPI in PBS or HBSS 15 minutes	5 μg/mL WGA-AF488 and 1 μg/mL DAPI in PBS or HBSS 4 hours to overnight
Wash	2 changes of 2 minutes each in PBS or HBSS	3 changes of 5 minutes each in PBS or HBSS
Mount	ProLong Gold or similar	ProLong Gold or similar

WGA-AF488 = Wheat Germ Agglutinin-Alexa Fluor 488 (Invitrogen, USA). *It is acceptable to wash for longer without adverse effects.