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. Author manuscript; available in PMC: 2016 Apr 6.
Published in final edited form as: J Biol Chem. 2007 Feb 6;282(16):12210–12219. doi: 10.1074/jbc.M610258200

FIGURE 3. Release of δ molecules in RRL is dependent on ISVP → ISVP* conversion.

FIGURE 3

A, SDS-PAGE and phosphorimaging: [35S]Met/Cys-labeled T1L virions, ISVPs, and ISVP*s prior to incubation with RRL reaction mixtures (RRL−) were compared with particles recovered after incubation with RRL (RRL+). B, SDS-PAGE and immunoblotting with anti-virion serum antibodies: T1L ISVPs were incubated at 37 °C in the presence of CsCl and at the indicated times, one aliquot was transferred to a tube with trypsin on ice and the other to a tube with RRL at 32 °C. Both incubations were for 45 min.