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. 2016 Jan 22;8(1):24–31. doi: 10.1038/ijos.2015.53

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Copyright © 2015 West China School of Stomatology

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Results of in situ hybridization on 16.5-day-old tooth germ. To verify the results of immunohistochemistry, we performed in situ hybridization, which confirmed the enhanced expression of Hsp 60 in the enamel organ and in odontoblasts. (a) Apical part of the enamel organ at a higher magnification; (b) proximal part; (c) labial root from the whole mount sample. The probe positively labelled the PreA, A, PreO, O, SI and OEE of the tooth germ. The IEE also shows a signal that is not very intense. Scale bar: 100 µm; a–c inserts, 20 µm. A, ameloblasts; IEE, enamel epithelium; O, odontoblasts; OEE, outer enamel epithelium; PreA, preameloblasts; PreO, preodontoblasts; SI, stratum intermedium.