Fig. 5.

Amplification bias. ExFold spike-in mixture 1 was added to 10 ng NT2 RNA, reverse transcribed and pre-amplified for 14 PCR cycles in a 355-plex PreAmp reaction. All 355 targets were quantified by qPCR using two technical replicates, the experiment was performed one time. Amplification bias was determined and plotted against target gene expression.