. 2015 Dec 21;6:13–21. doi: 10.1016/j.bdq.2015.12.001

Table 1.

Fold-change measurement error.

		Target gene expression level (Cq)
		<30	30–32.5	>32.5
No PreAmp	Average fold change measurement error (Cq)	0.093	0.265	0.804
	Average fold change measurement error (percent)	7%	20%	75%
88-plex PreAmp	Average fold change measurement error (Cq)	0.094	0.162	0.578
	Average fold change measurement error (percent)	7%	12%	49%
	Statistical equivalence with no PreAmp. p-value (upper threshold)	<0.0001^a	0.0121^a	0.0515
	Statistical equivalence with no PreAmp. p-value (lower threshold)	0.0021^a	0.0003^a	<0.0001^a
355-plex PreAmp	Average fold change measurement error (Cq)	0.108	0.119	0.380
	Average fold change measurement error (percent)	8%	9%	30%
	Statistical equivalence with no PreAmp. p-value (upper threshold)	<0.0001^a	0.0010^a	0.0074^a
	Statistical equivalence with no PreAmp. p-value (lower threshold)	0.0205^a	0.0050^a	0.0002^a
	Estimated fold-change limits-of-detection	2-fold	4-fold	8-fold

Statistically equivalent with no PreAmp.