Table 3.

Effect of PreAmp cycles on RNA detection.

PreAmp cycles	ERCC mix	PreAmp plex	Slope	R2	X-int (Cq)
0	Mix 1	NA	1.005	0.983	37.3
0	Mix 2	NA	0.988	0.984	36.9
10	Mix 1	88	0.995	0.979	34.4
10	Mix 1	355	0.994	0.978	34.5
10	Mix 2	88	0.987	0.979	34.1
10	Mix 2	355	0.985	0.980	34.1
12	Mix 1	88	0.996	0.979	32.3
12	Mix 1	355	0.993	0.980	32.4
12	Mix 2	88	0.989	0.978	32.0
12	Mix 2	355	0.983	0.979	32.4
14	Mix 1	88	0.996	0.977	30.3
14	Mix 1	355	0.991	0.975	30.3
14	Mix 2	88	1.000	0.977	30.3
14	Mix 2	355	0.987	0.978	30.1
16	Mix 1	88	1.009	0.976	28.5
16	Mix 1	355	0.994	0.972	28.4
16	Mix 2	88	0.992	0.978	28.2
16	Mix 2	355	0.994	0.979	28.2
18	Mix 1	88	0.998	0.969	26.3
18	Mix 1	355	0.999	0.966	26.4
18	Mix 2	88	0.997	0.969	26.2
18	Mix 2	355	1.000	0.971	26.2
20	Mix 1	88	1.013	0.964	24.3
20	Mix 1	355	1.010	0.958	24.5
20	Mix 2	88	1.010	0.960	24.4
20	Mix 2	355	1.021	0.960	24.1