Table 3.
PreAmp cycles | ERCC mix | PreAmp plex | Slope | R2 | X-int (Cq) |
---|---|---|---|---|---|
0 | Mix 1 | NA | 1.005 | 0.983 | 37.3 |
0 | Mix 2 | NA | 0.988 | 0.984 | 36.9 |
10 | Mix 1 | 88 | 0.995 | 0.979 | 34.4 |
10 | Mix 1 | 355 | 0.994 | 0.978 | 34.5 |
10 | Mix 2 | 88 | 0.987 | 0.979 | 34.1 |
10 | Mix 2 | 355 | 0.985 | 0.980 | 34.1 |
12 | Mix 1 | 88 | 0.996 | 0.979 | 32.3 |
12 | Mix 1 | 355 | 0.993 | 0.980 | 32.4 |
12 | Mix 2 | 88 | 0.989 | 0.978 | 32.0 |
12 | Mix 2 | 355 | 0.983 | 0.979 | 32.4 |
14 | Mix 1 | 88 | 0.996 | 0.977 | 30.3 |
14 | Mix 1 | 355 | 0.991 | 0.975 | 30.3 |
14 | Mix 2 | 88 | 1.000 | 0.977 | 30.3 |
14 | Mix 2 | 355 | 0.987 | 0.978 | 30.1 |
16 | Mix 1 | 88 | 1.009 | 0.976 | 28.5 |
16 | Mix 1 | 355 | 0.994 | 0.972 | 28.4 |
16 | Mix 2 | 88 | 0.992 | 0.978 | 28.2 |
16 | Mix 2 | 355 | 0.994 | 0.979 | 28.2 |
18 | Mix 1 | 88 | 0.998 | 0.969 | 26.3 |
18 | Mix 1 | 355 | 0.999 | 0.966 | 26.4 |
18 | Mix 2 | 88 | 0.997 | 0.969 | 26.2 |
18 | Mix 2 | 355 | 1.000 | 0.971 | 26.2 |
20 | Mix 1 | 88 | 1.013 | 0.964 | 24.3 |
20 | Mix 1 | 355 | 1.010 | 0.958 | 24.5 |
20 | Mix 2 | 88 | 1.010 | 0.960 | 24.4 |
20 | Mix 2 | 355 | 1.021 | 0.960 | 24.1 |