Fig. 1.

The original iPCR made use of a recombinant streptavidin-protein A chimera with bispecific affinity for DNA and antibodies to link linear plasmid DNA to an antibody specific for bovine serum albumin (BSA), which was immobilised on the surface of microtitre wells. Binding of the antibody to BSA resulted in a specific antigen–antibody–DNA conjugate that was detected by agarose gel electrophoresis after PCR amplification with plasmid-specific primers.