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. 2016 Apr 6;15:72. doi: 10.1186/s12944-016-0240-5

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© Tao et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — a Western blot detected the expression of Wnt signaling pathway key molecules. Hypoxia and reoxygenation (H+R) caused a marked increase in the protein levels of Dvl, β-catenin, and c-Myc. These changes were antagonized by Sfrp1-transfected cells before hypoxia and reoxygenation (Sfrp1+H+R); however, pre-treatment with the Wnt signaling pathway activator, Licl, increased the expression of Dvl, β-catenin, and c-Myc. b The median value of Dvl/GADPH with interquartile range (box) and range (whiskers). c The median value of β-catenin/GADPH with interquartile range (box) and range (whiskers). d The median value of c-Myc/GADPH with interquartile range (box) and range (whiskers). Post-hoc analyses of group differences were assessed with Kruskal-Wallis pairwise comparison. Statistical significance was set at the standard level (p<0.05), and the Bonferroni correction was applied in post-hoc analyses (α=0.05/6=0.0083). (Note:* vs. control P<0.001; # vs. H+R P<0.001; vs. Sfrp1+H+R P<0.001)