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. 2016 Apr;186(4):874–884. doi: 10.1016/j.ajpath.2015.11.022

Figure 1.

Figure 1

Fatty acids cause more cell death than their EEs. A and B: LDH leakage (A) and PI uptake (B) is increased compared with CONs in acini treated with unsaturated fatty acids LA and OA compared with LAEE and OAEE. C: LA and OA also cause decrease in ATP levels compared with their EEs. The saturated fatty acid PA does not cause any effect compared with its EE, PAEE. D: Western blot analysis for cytochrome c shows a leakage of cytochrome c from the M compartment to the C compartment in the presence of LA or OA. This intercompartment cytochrome c leakage is absent in LAEE and OAEE. Loading controls, α-tubulin for the C compartment and COX-IV for the M compartment, are equally present in all of the groups. Data are expressed as means ± SEM. ∗P < 0.05 versus CONs; †P < 0.05 versus LA/OA. ATP, adenosine triphosphate; C, cytosolic compartment; CON, control; COX, cytochrome c oxidase; EE, ethyl ester; LA linoleic acid; LDH, lactate dehydrogenase; M, mitochondrial compartment; OA, oleic acid; PA, palmitic acid; PI, propidium iodide.