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. Author manuscript; available in PMC: 2016 Apr 6.
Published in final edited form as: J Bone Miner Res. 2015 Oct 13;31(3):606–614. doi: 10.1002/jbmr.2723

Fig. 1.

Fig. 1

ERβ deletion in osteoprogenitor cells. Genomic DNA was isolated from either ex vivo bone marrow stromal cells (BMSCs) of the long bones of 12-week-old control and ERβPrx1–CKO mice cultured under osteogenic conditions or estrogen-responsive tissues harvested at the time of bone marrow isolation. DNA rearrangement of ERβ exon 3 was determined using primers that span exon 3. L = DNA ladder; C = control.